Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells

The therapeutic efficacy of mesenchymal stromal cells (MSCs) has been shown to rely on their immunomodulatory and regenerative properties. In order obtain sufficient numbers for clinical applications, MSCs have be expanded ex vivo. Expansion media with xenogeneic-free (XF) growth-promoting supplements like human platelet lysate (PL) or serum- (SF/XF) formulations established as safe efficient, both groups provide different beneficial qualities. this study, were in XF SF/XF well mixtures thereof. cultured these analyzed phenotypic functional MSC expansion was optimal conditions when PL present. Metabolic patterns, consumption growth factors, secretome differed depending the type concentration supplement. lactate per glucose yield increased along a higher proportion PL. Many factors supernatant showed distinct patterns supplement (e.g., FGF-2, TGFβ, insulin only PL-expanded MSC, leptin, sCD40L PDGF-AA SF/XF-expanded MSC). This also resulted changes cell characteristics migratory potential. These findings support current approaches where may utilized priming specific applications.